MHC Class I (RT1a) antibody

Catalog No. ABIN472144

This Mouse Monoclonal antibody specifically detects in FACS, IHC (fro), ELISA, IP and IHC. It exhibits reactivity toward Rat.

Quick Overview for MHC Class I (RT1a) antibody (ABIN472144)

Target: MHC Class I (RT1a)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Clone: OX-18

Product Details

Specificity: Recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognized. MRC OX-18 has been used in immunoaffinity purification of rat MHC class I molecules. This product is routinely tested in flow cytometry on rat splenocytes.

Purification: Affinity purified

Immunogen: Rat spleen glycoproteins.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo (1:50 - 1:100), IHC, IHC-Fr, IP

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. Immunohistology: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details

Target: MHC Class I (RT1a)

Alternative Name: MHC Class I RT1A